Part:BBa_K777125

Constitutive J23100 promoter

Fig. 1: Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)

This part is a twin of an Anderson promoter in K777133, a pSB1C3 derived backbone.

Usage and Biology

This construct can be useful in several ways, just like the similar partJ61002. However, if you plan to send coding or composite parts to the registry, they are already in the correct backbone here, pSB1C3.

1. You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites here.
2. Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites here.
3. It can provide a nice selection system for inserting your basic parts into the pSB1C3 vector via the EcoRI/XbaI and PstI site. If you use a construct containing a strong promoter e.g. K777125, colonies containing a self-ligated plasmid after transformation will turn red and you can select for the non-red colonies.

We also used this construct as a control for our swimming assays.

• Here is the complete sequence as an ApE file

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 8
  Illegal NheI site found at 31
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Fig. 2: Plasmid map of K777125